Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mecp2

Cell type

Cell type Class
Neural
Cell type
Frontal cortex
NA
NA

Attributes by original data submitter

Sample

source_name
Frontal Cortex
genotype
wild type
strain
C57BL/6
chip antibody
Total MeCP2 antibody (Chen et al. 2003)
age
6 weeks

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
total MeCP2 ChIP was performed as described in Cohen et al. Neuron 2012, Ebert et al Nature 2013 Single-end short read sequencing libraires were prepared using standard protocols, following the manufacturers instructions. Libraries were sequenced on the HiSeq 2500

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
54383677
Reads aligned (%)
97.2
Duplicates removed (%)
20.8
Number of peaks
609 (qval < 1E-05)

mm9

Number of total reads
54383677
Reads aligned (%)
96.8
Duplicates removed (%)
20.6
Number of peaks
693 (qval < 1E-05)

Base call quality data from DBCLS SRA